Microbiological detection for sustainable water use in chemical, paper, textile and food industries
Innovative technologies and services for sustainable water use in industries
In this report an overview is given about the up-to-date status on currently available microbiological data in the 4 different industries. The molecular-based methods used in the project for microbial analysis are summarized and the benefits and limitations of the technologies are discussed. The literature based information on micro-organisms supposed to be relevant in the four sectors is constantly updated and is substituted with new experimental information that has been achieved by applying suitable cultivation-dependent as well as cultivation-independent methods on samples from the paper and textile industry. Literature and experimental data were combined and a total of 184 micro-organisms were selected so far as being considered relevant for water streams within the four sectors. Based on the gained knowledge a prioritisation on further probe development can be performed. An update is provided on the current status of the gene probe development detecting pathogens and other micro-organisms supposed to be relevant for processes as well as in hygiene, biofilm and biofouling aspects. So far 62 gene probes are available for cultivation-independent monitoring of important micro-organisms directly in water samples of the four industries. As early biofilm forming bacteria in paper mills harboring neutral to alkaline conditions the first two bacteria groups are likely to be identified. The presence of bacteria related to Tepidimonas arfidensis and to Cloacibacterium as stable micro-flora in paper mill samples of SAP could be confirmed by using specific gene probes. Research is ongoing and if relevance can be confirmed the first easy-to-use monitoring kits for these organisms will be developed for general use. A first look into the black box of microbial diversity present in water samples from textile industry could be performed. By applying VIT-profiling and bio-digital approaches on samples from textile industry a surprising diversity of microbial populations could be analysed. All in all 79 isolates were identified and 205 clone sequences were analysed.